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Note: Following is a partial list of the recent publications containing results generated by ProtTech services.

Stawowczyk, M., Van Scoy, S, Kumar, K.M. and Reich, N.C. The Interferon Stimulated Gene 54 Promotes Apoptosis J. Biol. Chem. December 29, 2010, doi: 10.1074/jbc.M110.207068 [Abstract]

Gangaraju, V.K., Yin, H., Weiner, M.M., Wang, J., Huang, X.A. and Lin, H. Drosophila Piwi functions in Hsp90-mediated suppression of phenotypic variation Nature Genetics DOI: doi:10.1038/ng.743 (2010) [Abstract]

Kim, S.H., Shanware, N.P., Bowler, M. J. and Tibbetts, R.S. Amyotrophic Lateral Sclerosis-associated Proteins TDP-43 and FUS/TLS Function in a Common Biochemical Complex to Co-regulate HDAC6 mRNA J. Biol. Chem. 285: 34097-34105. (2010) [Abstract]

Zhou, G., Liu, Y., Wu, S.Y., Tie, F., Lou, H., Chiang, C.M. and Luo, G. Purification of a novel RECQL5-SWI/SNF-RNAPII super complex Int J Biochem Mol Biol. 1(1): 101-111 (2010) [Abstract]

Xu,Y., Boucher, J.M. and Morel, F.M.M. Expression and Diversity of Alkaline Phosphatase EHAP1 in Emiliania Huxleyi (Prymnesiophyceae)Journal of Phycology Volume 46, Issue 1, pages 85–92, (2010) [Abstract]

Chen, Y.W., Wang, J.K., Chou, F.P., Chen, C.Y., Rorke, E.A., Chen, L.M., Chai, K.X., Eckert, R.L., Johnson, M.D. and Lin, C.Y. Expand Regulation of the Matriptase-Prostasin Cell Surface Proteolytic Cascade by Hepatocyte Growth Factor Activator Inhibitor-1 during Epidermal Differentiation The Journal of Biological Chemistry, 285, 31755-31762 (2010) [Abstract]

Wilson, J.D. and Barlowe, B. Yet1p and Yet3p, the Yeast Homologs of BAP29 and BAP31, Interact with the Endoplasmic Reticulum Translocation Apparatus and Are Required for Inositol Prototrophy The Journal of Biological Chemistry, 285, 18252-18261 (2010) [Abstract]

El-Etr, S.H., Margolis, J.J., Monack, D., Robison, R.A. Cohen, M., Moore, E. and Rasley A. Francisella tularensis type A Strains Cause the Rapid Encystment of Acanthamoeba castellanii and Survive in Amoebal Cysts for Three Weeks post Infection Appl. Environ. Microbiol. December 75(23): 7488–7500 (2009) [Abstract]

Goo, Y.H. and Cooper T.A. CUGBP2 directly interacts with U2 17S snRNP components and promotes U2 snRNA binding to cardiac troponin T pre-mRNA Nucleic Acids Research, Jul. 37(13):4275-4286 (2009) [Abstract]

Lamango, N.S., Duverna, R., Zhang, W. and Ablordeppey, S.Y. Porcine Liver Carboxylesterase Requires Polyisoprenylation for High Affinity Binding to Cysteinyl Substrates Open Enzym Inhib J. 2009 January 1; 2: 12–27. (2009) [Abstract]

Yan, LJ and Forster MJ. Resolving mitochondrial protein complexes using nongradient blue native polyacrylamide gel electrophoresisAnalytical Biochemistry June 2009 Vol. 389(2):143-149 (2009) [Abstract]

Chan, SP and Slack, FJ. Ribosomal protein RPS-14 modulates let-7 microRNA function in Caenorhabditis elegans Developmental Biology Vol. 334, Issue 1, 1 October 2009, Pages 152-160 (2009) [Abstract]

Teijeiro, JM, Ignotz, GG and Marini, PE. Annexin A2 is involved in pig (Sus scrofa)sperm-oviduct interaction Molecular Reproduction and Development April 76(4):334-41 (2009) [Abstract]

Beller HR, Legler TC, Bourguet F, Letain TE, Kane SR and Coleman MA. Identification of c -type cytochromes involved in anaerobic, bacterial U(IV) oxidation. Biodegradation Volume 20, Number 1 / February, (2009) [Abstract]

Davie, JJ and Campagnari, AA. Comparative proteomic analysis of the Haemophilus ducreyi porin-deficient mutant 35000HP::P2AB. J. Bacteriol. April 2009, p. 2144-2152, Vol. 191, No. 7 (2009) [Abstract]

Jiz, M, Wu, HW, Meng, M, Pond-Tor,S, Reynolds, M, Friedman, JF, Olveda, R, Acosta, L and Kurtis, JD. Pilot-Scale Production and Characterization of Paramyosin, a Vaccine Candidate for Schistosomiasis Japonica. Infection and Immunity, July 2008, p. 3164-3169, Vol. 76, No. 7 (2008) [Abstract]

Hazelwood, LA, Free, RB, Cabrera, DM, Skinbjerg, M and Sibley, DR. Reciprocal Modulation of Function between the D1 and D2 Dopamine Receptors and the Na+,K+-ATPase. J. Biol. Chem., Vol. 283, Issue 52, 36441-36453, December 26, (2008) [Abstract]

Jiang, XR, Wang, P, Fu, X and Zhu, BT. Chemical synthesis and biochemical characterization of a biotinylated derivative of 17β-estradiol with a long side chain covalently attached to its C-7α position. Steroids Volume 73, Issue 12, November 2008, Pages 1252-1261 (2008)[Abstract]

Fu, X, Wang, P and Zhu, BT. Protein disulfide isomerase is a multifunctional regulator of estrogenic status in target cells. The Journal of Steroid Biochemistry and Molecular Biology Volume 112, Issues 1-3, November 2008, Pages 127-137 (2008) [Abstract]

Krawczyka, E, Hanoverb, JA, Schlegela, R and Suprynowicz, FA. Karyopherin β3: A new cellular target for the HPV-16 E5 oncoprotein.Biochemical and Biophysical Research Communications Volume 371, Issue 4, 11 July 2008, Pages 684-688 (2008) [Abstract]

Oh AS, Lahusen JT, Chien CD, Fereshteh MP,  Zhang X, Dakshanamurthy S, Xu, J,Kagan BL, Wellstein A and Riegel AT. Tyrosine phosphorylation of the nuclear receptor coactivator AIB1/SRC-3 is enhanced by Abl kinase and is required for its activity in cancer cells. Mol. Cell. Biol. November 2008, p. 6580-6593, Vol. 28, No. 21 (2008) [Abstract]

Adepu Kiran Kumara and Pranab Goswami. Purification and properties of a novel broad substrate specific alcohol oxidase from Aspergillus terreus MTCC 6324. Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics Volume 1784, Issue 11, November 2008, Pages 1552-1559 (2008) [Abstract]

Ozsvari B, Hegyi P, Sahin-Toth M. The Guinea Pig Pancreas Secretes a Single Trypsinogen Isoform, Which Is Defective in Autoactivation.Pancreas. 37(2):182-188, August (2008). [Abstract]

Loehfelm TW, Luke NR, and Campagnari AA. Identification and Characterization of an Acinetobacter baumannii Biofilm-Associated Protein. J. Bacteriology, 190(3), 1036-1044, (2008) [Abstract]

Patent WO/2007/005244 Henipavirus receptor and uses thereof, 11/1/2007 [Abstract]

Steffan RJ. Project Final Report: Biodegradation of 1,4-Dioxane. 8/1/2007 [Abstract]

North SH, Kirtland SE and Nakai H. Translation factor IF2 at the interface of transposition and replication by the PriA-PriC pathway. Molecular Microbiology 66 (6), 1566–1578. (2007) [Abstract]

United States Patent 20070128200, Growth arrest specific gene 6 peptides, antibodies, compositions, methods and uses, 06/07/2007[Abstract]

Chowdhury A., Mukhopadhyay J., Tharun S. The decapping activator Lsm1p-7p–Pat1p complex has the intrinsic ability to distinguish between oligoadenylated and polyadenylated RNAs. RNA 13:998-1016 (2007) [Abstract]

Free RB, Hazelwood LA, Namkung Y. Cabrera DM, Spalding HN, and Sibley DR. D1 and D2 Dopamine Receptor Expression is Regulated by Direct Interaction with the Chaperone Protein Calnexin. J. Biol. Chem., 282(29):21285-300 (2007) [Abstract]

Nusinow DA, Hernández-Muñoz I, Fazzio TG, Shah GM, Kraus WL, and Panning B. Poly(ADP-ribose) Polymerase 1 Is Inhibited by a Histone H2A Variant, MacroH2A, and Contributes to Silencing of the Inactive X Chromosome. J. Biol. Chem., 282(17), 12851-12859, (2007) [Abstract]

Plamondon P, Luke NR, Campagnari AA. Identification of a novel two-partner secretion locus in Moraxella catarrhalis. Infect. Immun., Vol. 75, No. 6, p. 2929-2936 (2007) [Abstract]

Shi Y, Dodson GE, Mukhopadhyay PS, Shanware NP, Trinh AT, Tibbetts RS. Identification of Carboxyl-terminal MCM3 Phosphorylation Sites Using Polyreactive Phosphospecific Antibodies. J. Biol. Chem., 282(12) 9236-9243 (2007) [Abstract]

Rickards B, Flint SJ, Cole MD, LeRoy G. Nucleolin Is Required for RNA Polymerase I Transcription In Vivo. Molecular and Cellular Biology, 27(3) 937-948 (2007) [Abstract]

Xu Y, Wahlund TM, Feng L, Shaked Y, Morel FMM. A Novel Alkaline Phosphatase In The Coccolithophore Emiliania Huxleyi (Prymnesiophyceae) And Its Regulation By Phosphorus. J. of Phycology 42 (4), 835–844 (2006) [Abstract]

Zeng G, Aldridge ME, Tian X, Seiler D, Zhang X, Jin Y, Rao J, Li W, Chen D, Langford MP, Duggan C, Belldegrun AS, Dubinett SM. Dendritic Cell Surface Calreticulin Is a Receptor for NY-ESO-1: Direct Interactions between Tumor-Associated Antigen and the Innate Immune System.The Journal of Immunology, 177: 3582-3589 (2006) [Abstract]

Kim BJ, Hood BL, Aragon RA, Hardwick JP, Conrads TP, Veenstra TD, Song BJ. Increased oxidation and degradation of cytosolic proteins in alcohol-exposed mouse liver and hepatoma cells. Proteomics. 6(4): 1250–1260 (2006) [Abstract]

Ono K, Yu R, Mohri K, Ono S. Caenorhabditis elegans Kettin, a Large Immunoglobulin-like Repeat Protein, Binds to Filamentous Actin and Provides Mechanical Stability to the Contractile Apparatuses in Body Wall Muscle. Mol Biol Cell. 17(6): 2722–2734 (2006) [Abstract]

Bernier M, Kwon YK, Pandey SK, Zhu TN, Zhao RJ, Maciuk A, He HJ, Decabo R, Kole S. Binding of manumycin A inhibits IkappaB kinase beta activityJ Biol Chem. 3;281(5):2551-61 (2006) [Abstract]

Ferraro D, Corso S, Fasano E, Panieri E, Santangelo R, Borrello S, Giordano S, Pani G, Galeotti T. Pro-metastatic signaling by c-Met through RAC-1 and reactive oxygen species (ROS). Oncogene. (2006) [Abstract]

Shi Y, Dodson GE, Shaikh S, Rundell K, Tibbetts RS. Ataxia-telangiectasia-mutated (ATM) Is a T-antigen Kinase That Controls SV40 Viral Replication in Vivo. J Biol Chem. 2;280(48):40195-200 (2005). [Abstract]

Fisher PW, Brigham-Burke M, Wu SJ, Luo J, Carton J, Staquet K, Gao W, Jackson S, Bethea D, Chen C, Hu B, Giles-Komar J, Yang J. A novel site contributing to growth-arrest-specific gene 6 binding to its receptors as revealed by a human monoclonal antibody. Biochem J. 1;387(Pt 3):727-35 (2005). [Abstract]

He L, and Lemasters JJ. Dephosphorylation of the Rieske iron-sulfur protein after induction of the mitochondrial permeability transition.Biochem Biophys Res Commun. 2;334(3):829-37 (2005). Rat mitochondrial membrane proteins were separated by 2-D gel and visualized by SyproRuby stainig. A differential spot in was identified by ProtTech using Nano LC-MS/MS. [Abstract]


Dendritic cell surface calreticulin is a receptor for NY-ESO-1: direct interactions between tumor-associated antigen and the innate immune system

G Zeng, ME Aldridge, X Tian, D Seiler et.al.Biochimie,Volume 94, Issue 1, January 2012, Pages 263–267

... Sections of SDS-gel were sliced and sent to ProtTech for the identification of specific proteins. ...

 Adsorption of cellular proteins to polyelectrolyte-functionalized gold nanorods: a mechanism for nanoparticle regulation of cell phenotype

PN Sisco, CG Wilson, D Chernak, JC Clark… -February 6, 2014  DOI: 10.1371/journal.pone.0086670

... To identify specific proteins bound to the nanorods, samples were sent to ProtTech (ProtTech,Norristown, PA) where they were reduced using dithiothreitol, alkylated with iodoacetamide (20 mM), trypsin digested, and analyzed by liquid chromatography-tandem mass .

 Searching for a placental derived ES-62-like molecule to explain rheumatoid arthritis amelioration in pregnancy

CD Scoville, D Rasmussen - Rheumatology Reports, 2014 - pagepress.org

... This same band was visualized using SDS-PAGE, stained with Imperial Protein Stain, and the band care- fully excised from the gel and sent to ProtTech Inc (Norristown, PA, USA) for protein sequenc- ing and identification using proprietary tech- nique and mass spectrometry. ...

 Identifying the ionically bound cell wall and intracellular glycoside hydrolases in late growth stage Arabidopsis stems: implications for the genetic engineering …

H Wei, R Brunecky, BS Donohoe, SY Ding… -Front Plant Sci. 2015; 6: 315.

... The above extracted Arabidopsis stem protein solution was shipped on dry ice to ProtTech Inc.(Phoenixville, PA, USA). ... This approach included the following five steps, mainly carried out at ProtTech Inc. (Phoenixville, PA, USA). Protein sample treatment and SDS-PAGE. ...

 Tumor necrosis factor α–mediated cleavage and inactivation of SirT1 in human osteoarthritic chondrocytes

M Dvir‐Ginzberg, V Gagarina, EJ Lee… - Arthritis & Rheumatism,Volume 63, Issue 8, pages 2363–2373, August 2011

 ... The extracts were then subjected to SDS-PAGE and stained with MagicBlue protein staining (MTR Scientific). The respective 75-kd bands were excised and processed for identification at ProtTech using NanoLC-MS/MS peptide sequencing technology. .

 Two dimensional blue native/SDS-PAGE to identify mitochondrial complex I subunits modified by 4-hydroxynonenal (HNE)

J Wu, X Luo, LJ Yan Frontiers in physiology, 2015

... al., 2000). Mass spectrometric peptide sequencing. Protein identification via mass spectrometric peptide sequencing was conducted at ProtTech (Phoneixville, PA) using NanoLC-MS/MS peptide sequencing technology. Briefly ..

 Cytomegalovirus vaccine strain towne-derived dense bodies induce broad cellular immune responses and neutralizing antibodies that prevent infection of fibroblasts …

C Cayatte, K Schneider-Ohrum, Z Wang… -J. Virol. October 2013 vol. 87 no. 20 11107-11120

... Subsequent mass spectrometry identifications were performed by ProtTech Inc. ... The mass spectrometric data were searched against the data in the nonredundant protein database (NR Database, NCBI) with ProtTech's ProtQuest software suite. ...

 Cellfree protein synthesis and purification of human dopamine D2 receptor long isoform

D Basu, JM Castellano, N Thomas… -Biotechnology Progress,Volume 29, Issue 3, pages 601–608, May/June 2013

... Coomassie blue. Bands which superimposed to the D2L band, as seen on the immunoblot, were excised with a stainless steel surgical blade around the expected MWT and sent for sequencing by ProtTech Inc. (Norristown, PA). Briefly ..

 Characterization of a unique motif in LIM mineralization protein-1 that interacts with jun activation-domain-binding protein 1

S Sangadala, K Yoshioka, Y Enyo, Y Liu, L Titus… -Molecular and Cellular Biochemistry,January 2014, Volume 385, Issue 1-2, pp 145-157

... Confirmation of protein identification was carried out at ProtTech, Inc ... The mass spectrometric data acquired were used to search the most recent nonredundant protein database from GenBank (http://www.ncbi.nlm.nih.gov/) with ProtTech's proprietary software suite. ...

 Stability of Structured Kaposi's Sarcoma-Associated Herpesvirus ORF57 Protein Is Regulated by Protein Phosphorylation and Homodimerization

V Majerciak, N Pripuzova, C Chan, N Temkin… -J. Virol. March 2015 vol. 89 no. 6 3256-3274

... Immunoprecipitated proteins were separated on SDS-PAGE and detected by Gel Blue staining or by Western blotting. The identity of immunoprecipitated proteins was determined by tandem mass spectrometry (MS/MS) after in-gel trypsin digestion (ProtTech, Norristown, PA). ...

 A protein isolated from human oviductal tissue in vitro secretion, identified as human lactoferrin, interacts with spermatozoa and oocytes and modulates …

CM Zumoffen, R Gil, AM Caille, C Morente… -Hum. Reprod. (2013) 28 (5): 1297-1308.

... Identifications of proteins in a gel slice containing the band with an estimated MW of 79 kDa was performed by nano liquid chromatography tandem mass spectrometry (LC-MS/MS) peptide sequencing technology using services provided by ProtTech (Norristown, PA, USA). ...

 Corinne Cayatte, Kirsten Schneider-Ohrum, Zhaoti Wang

GD Jin, AL McCormick, GH Liu, J Woo, G Van Nest… -Journal of Virology (Impact Factor: 4.65). 08/2013; 87.

... Subsequent mass spectrometry identifications were performed by ProtTech Inc. ... The massspectrometric data were searched against the data in the nonredundant protein database(NR Database, NCBI) with ProtTech's ProtQuest software suite. ..

 Robust protein nitration contributes to acetaminophen-induced mitochondrial dysfunction and acute liver injury

MA Abdelmegeed, S Jang, A Banerjee… -Free Radical Biology and Medicine,Volume 60, July 2013, Pages 211–222

... Mass-spectral analysis of affinity-purified nitrated proteins. Affinity-purified proteins were sent
for mass-spectral analysis performed by Dr. Drake Zhang at 
ProtTech (Germantown, PA, USA). ...
More experimental details can be found at www.
ProtTech.com. .

 Expression and purification of a natural N-terminal pre-ligand assembly domain of tumor necrosis factor receptor 1 (TNFR1 PLAD) and preliminary activity …

J Cao, F Meng, X Gao, H Dong, W Yao - The protein journal, 2011 - Springer

... 2.7 Nano LC–ESI–MS/MS The protein identification work was carried out at ProtTech,Inc. ... The mass spectrometric data acquired were used to search the most recent non-redundant protein database with ProtTech's proprietary software suite. ...

 Construction of Pichia pastoris Strains Constitutively Expressing Recombinant Human C-reactive Protein

J LI, H LIN, G GE, L ZHANG, F YU, X HU - Biotechnology, 2013 - en.cnki.com.cn

... 3, TIAN Xiao-mei REN Jian-hong FANG Cong(Radiology and Public Health School of Soochow University,Suzhou ProtTech Biotechnology Company Limited,Suzhou 215123,China);Proteomic Analysis of Pichia pastoris GS115 Cultured in Different Carbon Source Mediums[J ...

 Identification of staphylococcal nuclease domain-containing 1 (SND1) as a Metadherin-interacting protein with metastasis-promoting functions

MA Blanco, M Alečković, Y Hua, T Li, Y Wei… - The Journal of Biological Chemistry, 286, 19982-19992.

... Affinity purification of MTDH-GFP and subsequent mass spectrometry analysis were performed as described previously (30,–,32). LC-MS/MS-based identification of proteins isolated from bands sliced from silver-stained SDS-PAGE gels was performed by ProtTech, Inc. ...